I believe medical staff are aware that test strips play a significant role in medical testing. Regardless of which aspect they are used for, test strip production equipment is required in the early stages of production. Let's take a look at the relevant content.
Squeeze out the juice from tomato leaves in a dropper, dilute it with appropriate water, and then gently place it into a test strip. About 10 seconds later, two deep red stripes appeared clearly on the stripes, indicating that the leaves were infected with tomato brown wrinkled fruit virus (tobrfv).
This scene occurred in the Plant Virology Laboratory of Shandong Agricultural University. Recently, when reporters came to the laboratory for related reports, the laboratory director and plant virology expert professor told reporters, "This is the tomato brown wrinkled fruit virus detection test strip that our laboratory has just prepared. Compared with other detection methods, it has the advantages of high sensitivity, strong specificity, easy use, and simple operation. It is very suitable for on-site detection of a large number of samples and plays an important role in accurate monitoring and early warning of the virus. Tomato brown wrinkled fruit virus is a new virus that can be transmitted through seeds, contact, and bumblebees, seriously threatening tomato production safety. Tobrfv was first reported in Jordan and Israel, and subsequently appeared in countries such as the Americas, Europe, and Africa, attracting widespread attention from the international community. In this month of last year, many countries included tobrfv as a quarantine object, and it was also listed as a quarantine object in the "List of Harmful Organisms for Plant Quarantine in the People's Republic of China" jointly released by the Ministry of Agriculture and the General Administration of Customs.
Rapid identification and early warning are crucial for the prevention and control of tobrfv. The current detection methods for tobrfv include multiplex PCR and fluorescence quantification, both of which require precision instruments or professional operators. The colloidal gold immunoassay strip developed by the laboratory of plant virology experts is easy to operate and can be visually observed and judged within seconds or minutes, making it very suitable for on-site testing.
According to a plant virology expert professor, their laboratory used tobrfv particles as immunogens and prepared 17 monoclonal antibodies against tobrfv through hybridoma technology. Two types of monoclonal antibodies were immobilized on colloidal gold pads and nitrocellulose membranes for combination, and colloidal gold test strips were prepared to obtain 272 pairs of combinations. Select a set of paired antibody prepared test strips through specificity testing. This test strip can detect tobrfv from 12800 fold diluted tomato leaf disease juice (containing approximately 8 micrograms of tomato leaves), and it is fast and sensitive. But there is no response to other tobacco mosaic viruses, tomato mottle mosaic virus, and tomato mosaic virus, “
Meanwhile, the plant virology expert professor also said, "We conducted experiments on chili peppers and the results were equally satisfactory. Currently, this achievement has applied for a national invention patent and has been tested in agricultural technology promotion departments and enterprises in Shandong, Hebei, Henan, Jiangsu, Anhui and other places.
Based on the above content, the production of test strips through test strip production equipment has played a very important role in detecting tomato tobrfv.
